在免疫组化中用于检测兔源一抗的溶液。具有高灵敏度,仅一步,含有聚合物的检测试剂,特异性针对兔IgG。在石蜡包埋和冻存的组织中,它可以与所有过氧化物酶底物同时使用. 聚合物检测试剂消除了由于内源性生物素的存在而引起的假阳性染色,增强了IHC 检测的灵敏度。与传统的IHC 检测方法相比,所有产品仅需一步反应,且高度灵敏。SignalStain® IHC 增强型检测试剂对兔IgG是特异的,能够用于石蜡包埋切片或冰冻切片的显色,而且与所有过氧物酶底物匹配。
优点(与传统ABC方法比较):
出众的灵敏度,更强的信号,节约一抗(例如:一抗#4893的IHC稀释比例为 1:12800;一抗#3077的IHC稀释比例为1:161)。
减少检测过程的步骤,节约时间。
更少的 (由于内源性生物素染色引起的) 假阳性,更低的背景染色。
A ready-to-use solution intended for use in immunohistochemical assays to detect rabbit primary antibodies.It is a highly sensitive, one-step, polymer-based detection reagent that is specific for rabbit IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates. Some antibodies may require titration when used with this highly sensitive reagent. Directions for Use: Equilibrate SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) to room temperature prior to applying to the slides. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature. Wash slides and develop with any peroxidase-based substrate.
在免疫组化中用于检测小鼠源一抗的溶液。具有高灵敏度,仅一步,含有聚合物的检测试剂,特异性针对小鼠IgG。在石蜡包埋和冻存的组织中,它可以与所有过氧化物酶底物同时使用. 聚合物检测试剂消除了由于内源性生物素的存在而引起的假阳性染色,增强了IHC 检测的灵敏度。与传统的IHC 检测方法相比,所有产品仅需一步反应,且高度灵敏。SignalStain® IHC 增强型检测试剂对小鼠的IgG都是特异的,能够用于石蜡包埋切片或冰冻切片的显色,而且与所有过氧物酶底物匹配。
优点(与传统ABC方法比较):
出众的灵敏度,更强的信号,节约一抗(例如:一抗#4893的IHC稀释比例为 1:12800;一抗#3077的IHC稀释比例为1:160)。
减少检测过程的步骤,节约时间。
更少的 (由于内源性生物素染色引起的) 假阳性,更低的背景染色。
A ready-to-use solution intended for use in immunohistochemical assays to detect mouse primary antibodies.It is a highly sensitive, one-step, polymer-based detection reagent that is specific for mouse IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates. Some antibodies may require titration when used with this highly sensitive reagent. Directions for Use: Equilibrate SignalStain® Boost IHC Detection Reagent (HRP, Mouse) to room temperature prior to applying to the slides. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature. Wash slides and develop with any peroxidase-based substrate.
应用范围:WB(fluorescent),In-Cell Western
反应种属:mouse
标记:DyLight 800
Anti-mouse IgG (H+L) was conjugated to DyLight™ 800 4X PEG fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 777 nm and peak fluorescence emission is 794 nm.