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BrdU Cell Proliferation Chemiluminescent 分析试剂:BrdU Cell Proliferation Chemiluminescent Assa 1Kit

价格:¥

货号:5492S
品牌:CST

经销:基因生物技术国际贸易(上海)有限公司

BrdU细胞增殖化学发光检测试剂盒使用Anti-BrdU抗体检测细胞增殖时掺入DNA中的5-bromo-2’-deoxyuridine (BrdU)。使用含有BrdU的培养基培养细胞,这种嘧啶类似物能够在新DNA和成时取代胸腺嘧啶。移除培养基后,使用我们的固定/变性液以固定细胞,变性DNA。DNA变性能提高BrdU的掺入以便被抗体检测到。然后加入BrdU鼠单抗以检测掺入的BrdU。Anti-mouse IgG,HRP-偶联的抗体用以结合抗体。然后加入化学发光试剂。发射光的强度以相对光单位测得(RLU),与掺入细胞的BrdU量成正比,是细胞增殖的直接指示。BrdU细胞增殖化学发光检测试剂盒能够检测在细胞增殖时掺入细胞DNA的BrdU。BrdU标记的DNA必须经过变性才能使用试剂盒中的BrdU Mouse mAb检测。BrdU Mouse mAb与内源性DNA没有交叉反应。0.2-2x104 cells/well足以应对大多数实验类型,细胞数目取决于细胞类型和孵育时间。为了得到最好的结果,建议对细胞进行梯度稀释
500 assays (96 well format)。 The BrdU Cell Proliferation Assay Kit detects 5-bromo-2’-deoxyuridine (BrdU) incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are cultured with labeling medium that contains BrdU, this pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. After removing labeling medium, cells are fixed and the DNA is denatured with our fixing/denaturing solution. Denaturing of DNA is necessary to improve the accessibility of the incorporated BrdU to the detection antibody. A BrdU mouse mAb is then added to detect the incorporated BrdU. Anti-mouse IgG, HRP-linked Antibody is used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.
BrdU Cell Proliferation Chemiluminescent Assay Kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA must be denatured to be detected by the BrdU mouse mAb used in this kit. This BrdU mouse mAb does not cross-react with endogenous DNA. Depending on the cell type and the incubation time applied in the assay, 0.2-2x104 cells/well are sufficient for most experimental setups. For best results, a cell number titration (Figure 1) is recommended.

BrdU 细胞增殖分析试剂盒:BrdU Cell Proliferation Assay Kit 1Kit

价格:¥

货号:6813S
品牌:CST

经销:基因生物技术国际贸易(上海)有限公司

BrdU Cell Proliferation Assay kit能够检测细胞增殖过程中掺入到细胞DNA中的BrdU,使用的抗体为抗BrDU抗体。当细胞培养于含BrDU的标记培养基中,该胸腺嘧啶类似物可代替胸腺嘧啶掺入到增殖细胞的新合成DNA中。吸除标记培养基后,用固定/变性液固定细胞同时使DNA变性。然后加入BrdU鼠源单抗检测掺入的BrdU(DNA变性使得掺入的BrdU易于为抗体所识别)。Anti-mouse IgG, HRP-linked antibody用来识别联胞检测抗体。加入HRP底物(TMB)进行显色。显色过程中吸光度的强度和掺入细胞的BrdU的量成正比, BrdU是细胞增殖的直接证明。BrdU Cell Proliferation Assay kit能够检测细胞增殖过程中掺入到细胞DNA中的BrdU。BrDU标记的DNA变性后能够为试剂盒中的BrdU Mouse mAb检测到。BrdU Mouse mAb不与内源性DNA发生交叉反应。依细胞种类和孵育时间不同 ,每孔0.2-2x104个细胞即足够大部分实验。为了得到最佳实验结果,我们推荐进行细胞数滴度实验
500 assays (96 well format). The BrdU Cell Proliferation Assay Kit detects 5-bromo-2’-deoxyuridine (BrdU) incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are cultured with labeling medium that contains BrdU, this pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. After removing labeling medium, cells are fixed and the DNA is denatured with our fixing/denaturing solution. Then a BrdU mouse mAb is added to detect the incorporated BrdU (The denaturing of DNA is necessary to improve the accessibility of the incorporated BrdU to the detection antibody). Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.
BrdU Cell Proliferation Assay kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA has to be denatured to be detected by the BrdU Mouse mAb used in this kit. This BrdU Mouse mAb does not cross react with endogenous DNA. Depending on the cell type and the incubation time applied in the assay, 0.2-2x104 cells/well are sufficient for most experimental setups.

CCK-8 Cell Counting Kit 500 rxn

价格:¥

货号:A311-01
品牌:Vazyme

经销:南京诺唯赞生物

CCK-8 Cell Counting Kit, 简称CCK-8 试剂盒, 是依赖于WST-8 (2-(2- 甲氧基-4- 硝苯基)-3-(4- 硝苯基)-5-(2,4- 二磺基苯)-2H- 四唑单钠盐) 而广泛应用于细胞增殖和细胞毒性检测的快速、高灵敏度、无放射性的比色检测试剂盒。WST-8 在电子耦合载体1-Methoxy PMS 的作用下可被线粒体内的一些脱氢酶还原成高度水溶性的橙黄色甲臜产物(Formazan),生成的甲臜的颜色深浅与细胞增殖成正比,与细胞毒性成反比。 使用酶标仪在450 nm 波长处测得吸光值,该测定值可间接反映活细胞的数量。本试剂盒的CCK-8 Solution 为即用型试剂, 可直接加入到细胞样品中,孵育一定时间后即可检测,不需要预配各种成分。

CCK-8 Cell Counting Kit 1000 rxn

价格:¥

货号:A311-02
品牌:Vazyme

经销:南京诺唯赞生物

CCK-8 Cell Counting Kit, 简称CCK-8 试剂盒, 是依赖于WST-8 (2-(2- 甲氧基-4- 硝苯基)-3-(4- 硝苯基)-5-(2,4- 二磺基苯)-2H- 四唑单钠盐) 而广泛应用于细胞增殖和细胞毒性检测的快速、高灵敏度、无放射性的比色检测试剂盒。WST-8 在电子耦合载体1-Methoxy PMS 的作用下可被线粒体内的一些脱氢酶还原成高度水溶性的橙黄色甲臜产物(Formazan),生成的甲臜的颜色深浅与细胞增殖成正比,与细胞毒性成反比。 使用酶标仪在450 nm 波长处测得吸光值,该测定值可间接反映活细胞的数量。本试剂盒的CCK-8 Solution 为即用型试剂, 可直接加入到细胞样品中,孵育一定时间后即可检测,不需要预配各种成分。

ToxiLight™细胞毒性分析试剂盒(500次反应,有板):ToxiLight™ BioAssay Kit 500次反应

价格:¥

货号:LT17-217
品牌:Lonza

经销:基因生物技术国际贸易(上海)有限公司

Eu-TDA细胞毒试剂盒:DELFIA® EuTDA Cytotoxicity Reagents 10板*96

价格:¥

货号:AD0116
品牌:PerkinElmer

经销:基因生物技术国际贸易(上海)有限公司

Eu-Brdu细胞增殖试剂盒:DELFIA®Cell proliferation kit 10板*96

价格:¥

货号:AD0200
品牌:PerkinElmer

经销:基因生物技术国际贸易(上海)有限公司

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