All-round kit with highest sensitivity for purification of total DNA from clinical or forensic samples, tissues, cells, yeast, bacteria, or viruses • High DNA recovery and purity • Reliable DNA purification for reproducible results • Suitable for a huge variety of starting materials –more than 16 optimized support protocols available
Applications* • DNA from tissue (e.g., mouse tails) • DNA from cells (e.g., cultured human or animal cells, bacteria, yeast) • DNA from clinical samples (e.g., stool, urine, biopsy samples) • DNA from forensic samples (e.g., dried blood spots, hair, buccal swabs, cigarette stubs) • DNA from FFPE tissue • DNA from blood sample storage cards (e.g., NucleoCard®, page 145) • Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions NucleoSpin® Tissue Columns, Collection Tubes(2 mL), buffers, Proteinase K 10 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K
微量的DNA大片段快速回收浓缩，洗脱体积仅为6–10 µL Post clean-up and concentration of small amounts of large DNA fragments • Efficient post clean-up of large DNA fragments for successful downstream applications • Recommended for small DNA quantities extracted from, e.g., forensic samples • Unique XS spin column for low elution volume (> 6 µL) – highly concentrated DNA • Easier and faster than microdialysis filtration units • 20 min/6 preps – time-saving procedure
从组织和细胞中快速提取高浓度基因组DNA。5ul洗脱体积适用于微量样品。 Purification of genomic, bacterial, and viral DNA from smallest samples. • High sensitivity for DNA isolation from small sample quantities, like biopsy materials, forensic samples, or FACS sorted cells • Elution in as little as 5 μL resulting in highly concentrated DNA • Excellent DNA recovery and purity for reliable results • DNA ready to use for multiplex PCR and all common downstream applications
Applications* • DNA isolation from tissue (e.g., mouse kidney, laser micro-dissections) • DNA isolation from cells (e.g., bacteria, yeast, cultured cells) • DNA isolation from clinical samples (e.g., biopsy samples, fine needle aspirates) • DNA isolation from forensic samples (e.g., dried blood spots, buccal swabs, finger prints) • DNA suitable for sensitive applications (e.g., multiplex PCR, real-time PCR) NucleoSpin® Tissue XS Columns, Collection Tubes (2 mL), buffers, Proteinase K 10 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K
高分子量DNA的后继纯化浓缩。快速高效回收100 bp–50 kb DNA.不含裂解液。 Post clean-up and concentration of large sized DNA • Effective post clean-up and concentration of large-sized DNA for successful downstream applications • High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp • Concentrate your DNA sample easily and faster compared to microdialysis filtrations units • 15 min/10 preps – time-saving procedure
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 150 µL aqueous DNA solution (< 25 µg DNA) Fragment size: 100 bp–approx. 50 kbp Typical recovery: 80–90 % A260/A280: 1.8–1.9 Elution volume: 50–100 µL Preparation time: 15 min/10 preps Binding capacity: 50 µg Applications • Clean-up and concentration of pre-purified DNA (organic extractions) • Clean-up and concentration of DNA from enzymatic reactions • Not recommended for gel or PCR clean-up • Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification NucleoSpin® gDNA Clean-up Columns,Collection Tubes (2 mL), buffers 10 preps for clean-up of genomic DNA in aqueous solutions - NucleoSpin gDNA Clean-up Columns, Collection Tubes, buffers
快速从不超过25mg组织中提取高纯度基因组DNA。硅胶膜纯化柱最大载量60ug。操作简便，裂解后20分钟内完成提取，不同操作流程适合多种样品。For the rapid and efficient isolation of highly pure genomic DNA using the latest developments in silica membrane spin column technology. ISOLATE II Genomic DNA Kit is ideal for purification of highest quality DNA from a wide variety of starting materials such as mammalian tissue, paraffin embedded tissue, rodent tail, buccal swabs, bacteria, yeast and other eukaryotic cells.
Rapid protocol - ~20 minutes (after lysis)
High-purity genomic DNA - typical A260/A280 ratio 1.7 – 1.9
High yield - up to 35µg genomic DNA
Broad spectrum kit - with over 17 protocols
Applications Isolation of genomic DNA from:
Animal tissue and rodent tails
Paraffin embedded tissue
Clinical and forensic samples
Bacterial cells
Eukaryotic cells (yeast, cultured mammalian)
Downstream applications such as multiplex PCR, Southern blotting and real-time PCR.
Description
ISOLATE II Genomic DNA Kit is designed for the rapid and efficient isolation of highly pure genomic DNA from a variety of starting materials, such as mammalian tissue, paraffin embedded tissue, rodent tail, buccal swabs, bacteria, yeast and other eukaryotic cells. Over 17 optimized protocols are provided generating highly reproducible results with every sample.
ISOLATE II Genomic DNA Kit uses the latest developments in silica-membrane spin column technology. The isolation process combines fast lysis of the starting material with Proteinase K, followed by specific binding of DNA directly to the membrane in a spin column. Subsequent washing steps result in elution of high-quality DNA.
The online product manual has protocols for mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded tissue, stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgdorferi) and viral DNA (e.g. CMV) from urine, insects, dental swabs and buccal swabs.
The isolated DNA is suitable for downstream molecular biology applications such as multiplex PCR, Southern blotting, real-time PCR, sequencing, restriction analysis and microarrays.
高分子量DNA的后继纯化浓缩。快速高效回收100 bp–50 kb DNA.不含裂解液。 Post clean-up and concentration of large sized DNA • Effective post clean-up and concentration of large-sized DNA for successful downstream applications • High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp • Concentrate your DNA sample easily and faster compared to microdialysis filtrations units • 15 min/10 preps – time-saving procedure
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 150 µL aqueous DNA solution (< 25 µg DNA) Fragment size: 100 bp–approx. 50 kbp Typical recovery: 80–90 % A260/A280: 1.8–1.9 Elution volume: 50–100 µL Preparation time: 15 min/10 preps Binding capacity: 50 µg Applications • Clean-up and concentration of pre-purified DNA (organic extractions) • Clean-up and concentration of DNA from enzymatic reactions • Not recommended for gel or PCR clean-up • Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification NucleoSpin® gDNA Clean-up Columns,Collection Tubes (2 mL), buffers 50 preps for clean-up of genomic DNA in aqueous solutions - NucleoSpin gDNA Clean-up Columns, Collection Tubes, buffers
微量的DNA大片段快速回收浓缩，洗脱体积仅为6–10 µL Post clean-up and concentration of small amounts of large DNA fragments • Efficient post clean-up of large DNA fragments for successful downstream applications • Recommended for small DNA quantities extracted from, e.g., forensic samples • Unique XS spin column for low elution volume (> 6 µL) – highly concentrated DNA • Easier and faster than microdialysis filtration units • 20 min/6 preps – time-saving procedure
All-round kit with highest sensitivity for purification of total DNA from clinical or forensic samples, tissues, cells, yeast, bacteria, or viruses • High DNA recovery and purity • Reliable DNA purification for reproducible results • Suitable for a huge variety of starting materials –more than 16 optimized support protocols available
Applications* • DNA from tissue (e.g., mouse tails) • DNA from cells (e.g., cultured human or animal cells, bacteria, yeast) • DNA from clinical samples (e.g., stool, urine, biopsy samples) • DNA from forensic samples (e.g., dried blood spots, hair, buccal swabs, cigarette stubs) • DNA from FFPE tissue • DNA from blood sample storage cards (e.g., NucleoCard®, page 145) • Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions NucleoSpin® Tissue Columns, Collection Tubes(2 mL), buffers, Proteinase K 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K
从组织和细胞中快速提取高浓度基因组DNA。5ul洗脱体积适用于微量样品。 Purification of genomic, bacterial, and viral DNA from smallest samples. • High sensitivity for DNA isolation from small sample quantities, like biopsy materials, forensic samples, or FACS sorted cells • Elution in as little as 5 μL resulting in highly concentrated DNA • Excellent DNA recovery and purity for reliable results • DNA ready to use for multiplex PCR and all common downstream applications
Applications* • DNA isolation from tissue (e.g., mouse kidney, laser micro-dissections) • DNA isolation from cells (e.g., bacteria, yeast, cultured cells) • DNA isolation from clinical samples (e.g., biopsy samples, fine needle aspirates) • DNA isolation from forensic samples (e.g., dried blood spots, buccal swabs, finger prints) • DNA suitable for sensitive applications (e.g., multiplex PCR, real-time PCR) NucleoSpin® Tissue XS Columns, Collection Tubes (2 mL), buffers, Proteinase K 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K
快速从不超过25mg组织中提取高纯度基因组DNA。硅胶膜纯化柱最大载量60ug。操作简便，裂解后20分钟内完成提取，不同操作流程适合多种样品。For the rapid and efficient isolation of highly pure genomic DNA using the latest developments in silica membrane spin column technology. ISOLATE II Genomic DNA Kit is ideal for purification of highest quality DNA from a wide variety of starting materials such as mammalian tissue, paraffin embedded tissue, rodent tail, buccal swabs, bacteria, yeast and other eukaryotic cells.
Rapid protocol - ~20 minutes (after lysis)
High-purity genomic DNA - typical A260/A280 ratio 1.7 – 1.9
High yield - up to 35µg genomic DNA
Broad spectrum kit - with over 17 protocols
Applications Isolation of genomic DNA from:
Animal tissue and rodent tails
Paraffin embedded tissue
Clinical and forensic samples
Bacterial cells
Eukaryotic cells (yeast, cultured mammalian)
Downstream applications such as multiplex PCR, Southern blotting and real-time PCR.
Description
ISOLATE II Genomic DNA Kit is designed for the rapid and efficient isolation of highly pure genomic DNA from a variety of starting materials, such as mammalian tissue, paraffin embedded tissue, rodent tail, buccal swabs, bacteria, yeast and other eukaryotic cells. Over 17 optimized protocols are provided generating highly reproducible results with every sample.
ISOLATE II Genomic DNA Kit uses the latest developments in silica-membrane spin column technology. The isolation process combines fast lysis of the starting material with Proteinase K, followed by specific binding of DNA directly to the membrane in a spin column. Subsequent washing steps result in elution of high-quality DNA.
The online product manual has protocols for mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded tissue, stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgdorferi) and viral DNA (e.g. CMV) from urine, insects, dental swabs and buccal swabs.
The isolated DNA is suitable for downstream molecular biology applications such as multiplex PCR, Southern blotting, real-time PCR, sequencing, restriction analysis and microarrays.
高分子量DNA的后继纯化浓缩。快速高效回收100 bp–50 kb DNA.不含裂解液。 Post clean-up and concentration of large sized DNA • Effective post clean-up and concentration of large-sized DNA for successful downstream applications • High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp • Concentrate your DNA sample easily and faster compared to microdialysis filtrations units • 15 min/10 preps – time-saving procedure
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 150 µL aqueous DNA solution (< 25 µg DNA) Fragment size: 100 bp–approx. 50 kbp Typical recovery: 80–90 % A260/A280: 1.8–1.9 Elution volume: 50–100 µL Preparation time: 15 min/10 preps Binding capacity: 50 µg Applications • Clean-up and concentration of pre-purified DNA (organic extractions) • Clean-up and concentration of DNA from enzymatic reactions • Not recommended for gel or PCR clean-up • Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification NucleoSpin® gDNA Clean-up Columns,Collection Tubes (2 mL), buffers 250 preps for clean-up of genomic DNA in aqueous solutions - NucleoSpin gDNA Clean-up Columns, Collection Tubes, buffers
微量的DNA大片段快速回收浓缩，洗脱体积仅为6–10 µL Post clean-up and concentration of small amounts of large DNA fragments • Efficient post clean-up of large DNA fragments for successful downstream applications • Recommended for small DNA quantities extracted from, e.g., forensic samples • Unique XS spin column for low elution volume (> 6 µL) – highly concentrated DNA • Easier and faster than microdialysis filtration units • 20 min/6 preps – time-saving procedure
All-round kit with highest sensitivity for purification of total DNA from clinical or forensic samples, tissues, cells, yeast, bacteria, or viruses • High DNA recovery and purity • Reliable DNA purification for reproducible results • Suitable for a huge variety of starting materials –more than 16 optimized support protocols available
Applications* • DNA from tissue (e.g., mouse tails) • DNA from cells (e.g., cultured human or animal cells, bacteria, yeast) • DNA from clinical samples (e.g., stool, urine, biopsy samples) • DNA from forensic samples (e.g., dried blood spots, hair, buccal swabs, cigarette stubs) • DNA from FFPE tissue • DNA from blood sample storage cards (e.g., NucleoCard®, page 145) • Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions NucleoSpin® Tissue Columns, Collection Tubes(2 mL), buffers, Proteinase K 250 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K
从组织和细胞中快速提取高浓度基因组DNA。5ul洗脱体积适用于微量样品。 Purification of genomic, bacterial, and viral DNA from smallest samples. • High sensitivity for DNA isolation from small sample quantities, like biopsy materials, forensic samples, or FACS sorted cells • Elution in as little as 5 μL resulting in highly concentrated DNA • Excellent DNA recovery and purity for reliable results • DNA ready to use for multiplex PCR and all common downstream applications
Applications* • DNA isolation from tissue (e.g., mouse kidney, laser micro-dissections) • DNA isolation from cells (e.g., bacteria, yeast, cultured cells) • DNA isolation from clinical samples (e.g., biopsy samples, fine needle aspirates) • DNA isolation from forensic samples (e.g., dried blood spots, buccal swabs, finger prints) • DNA suitable for sensitive applications (e.g., multiplex PCR, real-time PCR) NucleoSpin® Tissue XS Columns, Collection Tubes (2 mL), buffers, Proteinase K 250 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K
快速从不超过25mg组织中提取高纯度基因组DNA。硅胶膜纯化柱最大载量60ug。操作简便，裂解后20分钟内完成提取，不同操作流程适合多种样品。For the rapid and efficient isolation of highly pure genomic DNA using the latest developments in silica membrane spin column technology. ISOLATE II Genomic DNA Kit is ideal for purification of highest quality DNA from a wide variety of starting materials such as mammalian tissue, paraffin embedded tissue, rodent tail, buccal swabs, bacteria, yeast and other eukaryotic cells.
Rapid protocol - ~20 minutes (after lysis)
High-purity genomic DNA - typical A260/A280 ratio 1.7 – 1.9
High yield - up to 35µg genomic DNA
Broad spectrum kit - with over 17 protocols
Applications Isolation of genomic DNA from:
Animal tissue and rodent tails
Paraffin embedded tissue
Clinical and forensic samples
Bacterial cells
Eukaryotic cells (yeast, cultured mammalian)
Downstream applications such as multiplex PCR, Southern blotting and real-time PCR.
Description
ISOLATE II Genomic DNA Kit is designed for the rapid and efficient isolation of highly pure genomic DNA from a variety of starting materials, such as mammalian tissue, paraffin embedded tissue, rodent tail, buccal swabs, bacteria, yeast and other eukaryotic cells. Over 17 optimized protocols are provided generating highly reproducible results with every sample.
ISOLATE II Genomic DNA Kit uses the latest developments in silica-membrane spin column technology. The isolation process combines fast lysis of the starting material with Proteinase K, followed by specific binding of DNA directly to the membrane in a spin column. Subsequent washing steps result in elution of high-quality DNA.
The online product manual has protocols for mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded tissue, stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgdorferi) and viral DNA (e.g. CMV) from urine, insects, dental swabs and buccal swabs.
The isolated DNA is suitable for downstream molecular biology applications such as multiplex PCR, Southern blotting, real-time PCR, sequencing, restriction analysis and microarrays.
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