通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等 Mini spin kit for the isolation of RNA of highest integrity • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion* • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included • Up to 70 μg ready-to-use RNA • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set • Also available for QIAcube®
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 5 x 10(6) cultured cells, < 10(9) bacterial cells, < 10(8) yeast cells, < 30 mg tissue Fragment size: > 200 b Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells A260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 40–120 µL Preparation time: 30 min/6 preps Binding capacity: 200 µg
Applications** • Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells • Support protocol for total RNA from ≤ 100 μL biological fluids • Support protocol for RNA clean-up from reaction mixtures • Support protocol for total RNA from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with: – the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid – decreasing PCR amplicon size NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 10 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA • Isolation of RNA from small sample quantities like biopsy material or single cells • Excellent RNA recovery and integrity • Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL • rDNase included for on-column DNA removal • Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included • High quality RNA, ready to use for RT-PCR and other applications
Technology: Silica-membrane technology Format: Mini spin column – XS design Sample material: Small amounts of tissue < 5 mg, 1-100000 cultured cells Fragment size: > 200 b Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ng A260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 (depending on sample quality) Elution volume: 5–30 µL Preparation time: 40 min/12 preps Binding capacity: 110 µg
Applications* • Total RNA isolation from cultured cells • Total RNA isolation from tissue • Total RNA isolation from cryosections • Total RNA isolation from laser captured cells • Total RNA isolation from small amounts of plant material • Total RNA isolation from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP 10 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
从组织和细胞中快速提取6-700ug的更完整的RNA。离心柱式，载量达700ug。提供NucleoSpin® Filters Midi (shredders)有效降低裂解液粘稠度以均质化。提供rDNase实现纯化柱上的DNA降解，有效去除DNA污染。 Midi spin kit for the isolation of RNA of highest integrity • Efficient removal of genomic DNA – rDNase included for on-column digestion* • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters Midi (shredders) included • Up to 600 μg ready-to-use RNA
Technology: Silica-membrane technology Format: Midi spin columns Sample material: < 5 x 10(7) cultured cells, < 10(10) bacterial cells, < 3 x 10(8) yeast cells, < 200 mg tissue Fragment size: 200 b Typical yield: 180 µg from 10(7) HeLa cells, 620 µg from 4 x 10(7) HeLa cells A260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 500 µL Preparation time: 80 min/4 preps Binding capacity: 700 µg
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA Midi system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with: – the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid – decreasing PCR amplicon size NucleoSpin® RNA Midi Columns with Collection Tubes(15 mL), Collection Tubes (15 mL), NucleoSpin® Filters Midi, buffers, RNase-free rDNase 20 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA L Columns, NucleoSpin RNA L Filters, Elution Tubes, buffers, RNase-free rDNase
通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等 Mini spin kit for the isolation of RNA of highest integrity • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion* • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included • Up to 70 μg ready-to-use RNA • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set • Also available for QIAcube®
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 5 x 10(6) cultured cells, < 10(9) bacterial cells, < 10(8) yeast cells, < 30 mg tissue Fragment size: > 200 b Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells A260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 40–120 µL Preparation time: 30 min/6 preps Binding capacity: 200 µg
Applications** • Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells • Support protocol for total RNA from ≤ 100 μL biological fluids • Support protocol for RNA clean-up from reaction mixtures • Support protocol for total RNA from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with: – the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid – decreasing PCR amplicon size NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 50 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA • Isolation of RNA from small sample quantities like biopsy material or single cells • Excellent RNA recovery and integrity • Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL • rDNase included for on-column DNA removal • Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included • High quality RNA, ready to use for RT-PCR and other applications
Technology: Silica-membrane technology Format: Mini spin column – XS design Sample material: Small amounts of tissue < 5 mg, 1-100000 cultured cells Fragment size: > 200 b Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ng A260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 (depending on sample quality) Elution volume: 5–30 µL Preparation time: 40 min/12 preps Binding capacity: 110 µg
Applications* • Total RNA isolation from cultured cells • Total RNA isolation from tissue • Total RNA isolation from cryosections • Total RNA isolation from laser captured cells • Total RNA isolation from small amounts of plant material • Total RNA isolation from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP 50 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等 Mini spin kit for the isolation of RNA of highest integrity • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion* • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included • Up to 70 μg ready-to-use RNA • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set • Also available for QIAcube®
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 5 x 10(6) cultured cells, < 10(9) bacterial cells, < 10(8) yeast cells, < 30 mg tissue Fragment size: > 200 b Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells A260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 40–120 µL Preparation time: 30 min/6 preps Binding capacity: 200 µg
Applications** • Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells • Support protocol for total RNA from ≤ 100 μL biological fluids • Support protocol for RNA clean-up from reaction mixtures • Support protocol for total RNA from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with: – the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid – decreasing PCR amplicon size NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 250 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA • Isolation of RNA from small sample quantities like biopsy material or single cells • Excellent RNA recovery and integrity • Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL • rDNase included for on-column DNA removal • Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included • High quality RNA, ready to use for RT-PCR and other applications
Technology: Silica-membrane technology Format: Mini spin column – XS design Sample material: Small amounts of tissue < 5 mg, 1-100000 cultured cells Fragment size: > 200 b Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ng A260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 (depending on sample quality) Elution volume: 5–30 µL Preparation time: 40 min/12 preps Binding capacity: 110 µg
Applications* • Total RNA isolation from cultured cells • Total RNA isolation from tissue • Total RNA isolation from cryosections • Total RNA isolation from laser captured cells • Total RNA isolation from small amounts of plant material • Total RNA isolation from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP 250 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
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