通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等Mini spin kit for the isolation of RNA of highest integrity • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included• Up to 70 μg ready-to-use RNA• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set• Also available for QIAcube® Technology: Silica-membrane technology Format: Mini spin columns Sample material: Fragment size: > 200 b Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cellsA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 40–120 µL Preparation time: 30 min/6 preps Binding capacity: 200 µg Applications** • Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from • Support protocol for total RNA from ≤ 100 μL biological fluids• Support protocol for RNA clean-up from reaction mixtures• Support protocol for total RNA from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays * The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:– the number of DNA copies per preparation: single copy target – decreasing PCR amplicon sizeNucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 10 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

从微量样品如单细胞、活检样品中提取高浓缩的RNA。Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA • Isolation of RNA from small sample quantities like biopsy material or single cells• Excellent RNA recovery and integrity• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL• rDNase included for on-column DNA removal• Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included• High quality RNA, ready to use for RT-PCR and other applications Technology: Silica-membrane technology Format: Mini spin column – XS design Sample material: Small amounts of tissue Fragment size: > 200 b Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ngA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 (depending on sample quality) Elution volume: 5–30 µL Preparation time: 40 min/12 preps Binding capacity: 110 µg Applications* • Total RNA isolation from cultured cells• Total RNA isolation from tissue• Total RNA isolation from cryosections• Total RNA isolation from laser captured cells• Total RNA isolation from small amounts of plant material• Total RNA isolation from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP 10 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP

从组织和细胞中快速提取6-700ug的更完整的RNA。离心柱式，载量达700ug。提供NucleoSpin® Filters Midi (shredders)有效降低裂解液粘稠度以均质化。提供rDNase实现纯化柱上的DNA降解，有效去除DNA污染。Midi spin kit for the isolation of RNA of highest integrity • Efficient removal of genomic DNA – rDNase included for on-column digestion*• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters Midi (shredders) included• Up to 600 μg ready-to-use RNA Technology: Silica-membrane technology Format: Midi spin columns Sample material: Fragment size: 200 b Typical yield: 180 µg from 10(7) HeLa cells, 620 µg from 4 x 10(7) HeLa cellsA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 500 µL Preparation time: 80 min/4 preps Binding capacity: 700 µg * The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA Midi system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:– the number of DNA copies per preparation: single copy target – decreasing PCR amplicon sizeNucleoSpin® RNA Midi Columns with Collection Tubes(15 mL), Collection Tubes (15 mL), NucleoSpin® Filters Midi, buffers, RNase-free rDNase 20 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA L Columns, NucleoSpin RNA L Filters, Elution Tubes, buffers, RNase-free rDNase

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等Mini spin kit for the isolation of RNA of highest integrity • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included• Up to 70 μg ready-to-use RNA• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set• Also available for QIAcube® Technology: Silica-membrane technology Format: Mini spin columns Sample material: Fragment size: > 200 b Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cellsA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 40–120 µL Preparation time: 30 min/6 preps Binding capacity: 200 µg Applications** • Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from • Support protocol for total RNA from ≤ 100 μL biological fluids• Support protocol for RNA clean-up from reaction mixtures• Support protocol for total RNA from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays * The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:– the number of DNA copies per preparation: single copy target – decreasing PCR amplicon sizeNucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 50 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

从微量样品如单细胞、活检样品中提取高浓缩的RNA。操作简单，RNA回收率高，完整性好，洗脱体积仅需5ul。提供NucleoSpin® Filters (shredders)有效降低裂解液粘度和均质化。Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA • Isolation of RNA from small sample quantities like biopsy material or single cells• Excellent RNA recovery and integrity• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL• rDNase included for on-column DNA removal• Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included• High quality RNA, ready to use for RT-PCR and other applications Technology: Silica-membrane technology Format: Mini spin column – XS design Sample material: Small amounts of tissue Fragment size: > 200 b Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ngA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 (depending on sample quality) Elution volume: 5–30 µL Preparation time: 40 min/12 preps Binding capacity: 110 µg Applications* • Total RNA isolation from cultured cells• Total RNA isolation from tissue• Total RNA isolation from cryosections• Total RNA isolation from laser captured cells• Total RNA isolation from small amounts of plant material• Total RNA isolation from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP 50 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等Mini spin kit for the isolation of RNA of highest integrity • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included• Up to 70 μg ready-to-use RNA• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set• Also available for QIAcube® Technology: Silica-membrane technology Format: Mini spin columns Sample material: Fragment size: > 200 b Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cellsA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 Elution volume: 40–120 µL Preparation time: 30 min/6 preps Binding capacity: 200 µg Applications** • Total RNA isolation from cultured cells, tissue (standard protocol) • Support protocol for total RNA from • Support protocol for total RNA from ≤ 100 μL biological fluids• Support protocol for RNA clean-up from reaction mixtures• Support protocol for total RNA from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays * The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:– the number of DNA copies per preparation: single copy target – decreasing PCR amplicon sizeNucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase 250 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

从微量样品如单细胞、活检样品中提取高浓缩的RNA。操作简单，RNA回收率高，完整性好，洗脱体积仅需5ul。提供NucleoSpin® Filters (shredders)有效降低裂解液粘度和均质化。Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA • Isolation of RNA from small sample quantities like biopsy material or single cells• Excellent RNA recovery and integrity• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL• rDNase included for on-column DNA removal• Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included• High quality RNA, ready to use for RT-PCR and other applications Technology: Silica-membrane technology Format: Mini spin column – XS design Sample material: Small amounts of tissue Fragment size: > 200 b Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ngA260/A280: 1.9–2.1 Typical RIN (RNA integrity number): > 9 (depending on sample quality) Elution volume: 5–30 µL Preparation time: 40 min/12 preps Binding capacity: 110 µg Applications* • Total RNA isolation from cultured cells• Total RNA isolation from tissue• Total RNA isolation from cryosections• Total RNA isolation from laser captured cells• Total RNA isolation from small amounts of plant material• Total RNA isolation from samples stored in RNAlater® • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP 250 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP