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生物通商城首页 -> 试剂 > RNA分析 > RNA纯化 > 通用快速离心式总RNA纯化

RNA分析

RNAi

miRNA

RNA纯化

RNA保护试剂

RNAse抑制剂

RNase

RNase污染检测

反义RNA

去除环境RNase

RNA合成/体外转录

体外转录+翻译

体外翻译

反转录/cDNA合成

RNA FISH探针/荧光示踪

lncRNA分析

随机引物

Northern Blot

各类细胞(RNAlater保存/提取RNA)

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产品名/货号/品牌 简介 包装  
NucleoSpin RNA 提取试剂盒:NucleoSpin RNA 10 preps

价格:¥

货号:740955.10
品牌:MNG

经销:基因有限公司

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化,柱上进行DNA消化可有效去除DNA污染,还可平行纯化基因组DNA。柱载量200ug,洗脱体积40-120ul,适用于组织,培养细胞,细菌和酵母等
Mini spin kit for the isolation of RNA of highest integrity
• Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set
• Also available for QIAcube®

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: < 5 x 10(6) cultured cells, < 10(9) bacterial cells, < 10(8) yeast cells, < 30 mg tissue
Fragment size: > 200 b
Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9
Elution volume: 40–120 µL
Preparation time: 30 min/6 preps
Binding capacity: 200 µg

Applications**
• Total RNA isolation from cultured cells, tissue (standard protocol)
• Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• Support protocol for total RNA from ≤ 100 μL biological fluids
• Support protocol for RNA clean-up from reaction mixtures
• Support protocol for total RNA from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
10 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin RNA 提取试剂盒 Midi:NucleoSpin RNA Midi 20 ml

价格:¥

货号:740962.20
品牌:MNG

经销:基因有限公司

从组织和细胞中快速提取6-700ug的最完整的RNA。离心柱式,载量达700ug。提供NucleoSpin® Filters Midi (shredders)有效降低裂解液粘稠度以均质化。提供rDNase实现纯化柱上的DNA降解,有效去除DNA污染。
Midi spin kit for the isolation of RNA of highest integrity
• Efficient removal of genomic DNA – rDNase included for on-column digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters Midi (shredders)
included
• Up to 600 μg ready-to-use RNA

Technology: Silica-membrane technology
Format: Midi spin columns
Sample material: < 5 x 10(7) cultured cells, < 10(10) bacterial cells, < 3 x 10(8) yeast cells, < 200 mg tissue
Fragment size: 200 b
Typical yield: 180 µg from 10(7) HeLa cells, 620 µg from 4 x 10(7) HeLa cells
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9
Elution volume: 500 µL
Preparation time: 80 min/4 preps
Binding capacity: 700 µg

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA Midi system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
NucleoSpin® RNA Midi Columns with Collection Tubes(15 mL), Collection Tubes (15 mL), NucleoSpin® Filters Midi, buffers, RNase-free rDNase
20 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA L Columns, NucleoSpin RNA L Filters, Elution Tubes, buffers, RNase-free rDNase

NucleoSpin RNA 提取试剂盒:NucleoSpin RNA 50 preps

价格:¥

货号:740955.50
品牌:MNG

经销:基因有限公司

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化,柱上进行DNA消化可有效去除DNA污染,还可平行纯化基因组DNA。柱载量200ug,洗脱体积40-120ul,适用于组织,培养细胞,细菌和酵母等
Mini spin kit for the isolation of RNA of highest integrity
• Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set
• Also available for QIAcube®

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: < 5 x 10(6) cultured cells, < 10(9) bacterial cells, < 10(8) yeast cells, < 30 mg tissue
Fragment size: > 200 b
Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9
Elution volume: 40–120 µL
Preparation time: 30 min/6 preps
Binding capacity: 200 µg

Applications**
• Total RNA isolation from cultured cells, tissue (standard protocol)
• Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• Support protocol for total RNA from ≤ 100 μL biological fluids
• Support protocol for RNA clean-up from reaction mixtures
• Support protocol for total RNA from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
50 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

NucleoSpin RNA 提取试剂盒:NucleoSpin RNA 250 preps

价格:¥

货号:740955.250
品牌:MNG

经销:基因有限公司

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化,柱上进行DNA消化可有效去除DNA污染,还可平行纯化基因组DNA。柱载量200ug,洗脱体积40-120ul,适用于组织,培养细胞,细菌和酵母等
Mini spin kit for the isolation of RNA of highest integrity
• Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set
• Also available for QIAcube®

Technology: Silica-membrane technology
Format: Mini spin columns
Sample material: < 5 x 10(6) cultured cells, < 10(9) bacterial cells, < 10(8) yeast cells, < 30 mg tissue
Fragment size: > 200 b
Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells
A260/A280: 1.9–2.1
Typical RIN (RNA integrity number): > 9
Elution volume: 40–120 µL
Preparation time: 30 min/6 preps
Binding capacity: 200 µg

Applications**
• Total RNA isolation from cultured cells, tissue (standard protocol)
• Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• Support protocol for total RNA from ≤ 100 μL biological fluids
• Support protocol for RNA clean-up from reaction mixtures
• Support protocol for total RNA from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
RNase protection assays

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
250 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

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