Bst DNA 聚合酶/大片段用于DNA 等温扩增，富含 GC 区域的DNA 测序，纳克级含量 DNA 模板的快速测序，可用于要求嗜温链置换的实验。具有5´ →3´DNA 聚合酶活性，但缺失5´ →3´核酸外切酶活性。建议反应温度不要超过 70℃。Bst DNA 聚合酶，大片段不能用于热循环测序或 PCR。

全长的Bst DNA聚合酶具有5´→3´聚合酶活性和双链特异的5´→3´核酸外切酶活性，但缺失3´→5´核酸外切酶活性。用于 DNA 等温扩增， 引物延伸。建议反应温度不要超过 70℃。Bst DNA 聚合酶，全长不能用于热循环测序或 PCR。

phi29 DNA 聚合酶是从噬菌体 phi29 中克隆出的 DNA 聚合酶。此酶具有卓越的链置换和连续合成特性，并具有 3´ →5´ 核酸外切酶校读活性。极高的掺入率，超强的链置换能力，应用于需要强链置换和/或连续合成的复制反应，中温条件下高保真复制

Bst DNA 聚合酶/大片段用于DNA 等温扩增，富含 GC 区域的DNA 测序，纳克级含量 DNA 模板的快速测序，可用于要求嗜温链置换的实验。具有5´ →3´DNA 聚合酶活性，但缺失5´ →3´核酸外切酶活性。建议反应温度不要超过 70℃。Bst DNA 聚合酶，大片段不能用于热循环测序或 PCR。

Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity. Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.Applications:Isothermal amplification (LAMP, RTLAMP);Applications requiring strand-displacement DNA synthesis;Reverse transcription at elevated temperature (to 72°C);Amplification in the presence of impure samples or amplification inhibitors;

等温聚合酶Bst 2.0 DNA 聚合酶是Bst DNA 聚合酶,大片段的同源物，并由电脑模拟设计完成。Bst 2.0 DNA 聚合酶具有 5´→3´ 的聚合酶活性和强链置换活性，但没有 5´→3´ 核酸外切酶活性。有效提高扩增速度、产量、耐盐性和热稳定性等。反应条件灵活，比 Bst DNA 聚合酶具有更高的盐耐受力，用 dUTP 替换 dTTP 对其几乎无影响

等温聚合酶Bst 2.0 WarmStart DNA 聚合酶像“热启动” PCR 聚合酶一样，利用了核酸适配体技术，在非许可温度下抑制酶活性（＜ 50℃）。因此，可以消除在室温条件下建立反应时产生的非特异性的扩增，提高反应效率。不需要单独的激活步骤。具有 5´→3´ 的聚合酶活性和强链置换活性，但没有 5´→3´ 核酸外切酶活性。有效提高扩增速度、产量、耐盐性和热稳定性等。反应条件灵活，比 Bst DNA 聚合酶具有更高的盐耐受力，用 dUTP 替换 dTTP 对其几乎无影响

（LAMP）环介导等温扩增反应是一种核酸扩增技术，广泛用于目标 DNA 或 RNA 的检测。NEB的 WarmStart® LAMP 产品线为检测目标 DNA 或 RNA提供简单、一步法的解决方案。试剂盒中提供预混液，混合有快速、强力的 Bst 2.0 WarmStart® DNA 聚合酶 和 WarmStart RTx 反转录酶，两种酶均经过基因工程改造，提高了 LAMP 反应的效率。试剂盒还提供了一种荧光染料以便于在 LAMP 反应中进行实时荧光检测。WarmStart LAMP 试剂盒可以兼容多种检测方法，包括浊度检测、实时荧光检测（当使用灯荧光染料时）和可视化终点法。The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.

The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes. 

Bst DNA 聚合酶/大片段用于DNA 等温扩增，富含 GC 区域的DNA 测序，纳克级含量 DNA 模板的快速测序，可用于要求嗜温链置换的实验。具有5´ →3´DNA 聚合酶活性，但缺失5´ →3´核酸外切酶活性。建议反应温度不要超过 70℃。Bst DNA 聚合酶，大片段不能用于热循环测序或 PCR。

Bst DNA 聚合酶/大片段用于DNA 等温扩增，富含 GC 区域的DNA 测序，纳克级含量 DNA 模板的快速测序，可用于要求嗜温链置换的实验。具有5´ →3´DNA 聚合酶活性，但缺失5´ →3´核酸外切酶活性。建议反应温度不要超过 70℃。Bst DNA 聚合酶，大片段不能用于热循环测序或 PCR。

phi29 DNA 聚合酶是从噬菌体 phi29 中克隆出的 DNA 聚合酶。此酶具有卓越的链置换和连续合成特性，并具有 3´ →5´ 核酸外切酶校读活性。极高的掺入率，超强的链置换能力，应用于需要强链置换和/或连续合成的复制反应，中温条件下高保真复制

等温聚合酶Bst 2.0 DNA 聚合酶是Bst DNA 聚合酶,大片段的同源物，并由电脑模拟设计完成。Bst 2.0 DNA 聚合酶具有 5´→3´ 的聚合酶活性和强链置换活性，但没有 5´→3´ 核酸外切酶活性。有效提高扩增速度、产量、耐盐性和热稳定性等。反应条件灵活，比 Bst DNA 聚合酶具有更高的盐耐受力，用 dUTP 替换 dTTP 对其几乎无影响

Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity. Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.Applications:Isothermal amplification (LAMP, RTLAMP);Applications requiring strand-displacement DNA synthesis;Reverse transcription at elevated temperature (to 72°C);Amplification in the presence of impure samples or amplification inhibitors;

Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity. Bst 3.0 DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity. Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.Applications:Isothermal amplification (LAMP, RTLAMP);Applications requiring strand-displacement DNA synthesis;Reverse transcription at elevated temperature (to 72°C);Amplification in the presence of impure samples or amplification inhibitors;

等温聚合酶Bst 2.0 DNA 聚合酶是Bst DNA 聚合酶,大片段的同源物，并由电脑模拟设计完成。Bst 2.0 DNA 聚合酶具有 5´→3´ 的聚合酶活性和强链置换活性，但没有 5´→3´ 核酸外切酶活性。有效提高扩增速度、产量、耐盐性和热稳定性等。反应条件灵活，比 Bst DNA 聚合酶具有更高的盐耐受力，用 dUTP 替换 dTTP 对其几乎无影响

等温聚合酶Bst 2.0 WarmStart DNA 聚合酶像“热启动” PCR 聚合酶一样，利用了核酸适配体技术，在非许可温度下抑制酶活性（＜ 50℃）。因此，可以消除在室温条件下建立反应时产生的非特异性的扩增，提高反应效率。不需要单独的激活步骤。具有 5´→3´ 的聚合酶活性和强链置换活性，但没有 5´→3´ 核酸外切酶活性。有效提高扩增速度、产量、耐盐性和热稳定性等。反应条件灵活，比 Bst DNA 聚合酶具有更高的盐耐受力，用 dUTP 替换 dTTP 对其几乎无影响

等温聚合酶Bst 2.0 WarmStart DNA 聚合酶像“热启动” PCR 聚合酶一样，利用了核酸适配体技术，在非许可温度下抑制酶活性（＜ 50℃）。因此，可以消除在室温条件下建立反应时产生的非特异性的扩增，提高反应效率。不需要单独的激活步骤。具有 5´→3´ 的聚合酶活性和强链置换活性，但没有 5´→3´ 核酸外切酶活性。有效提高扩增速度、产量、耐盐性和热稳定性等。反应条件灵活，比 Bst DNA 聚合酶具有更高的盐耐受力，用 dUTP 替换 dTTP 对其几乎无影响

热解旋酶扩增法（tHDA）是一种在恒温条件下扩增核酸的新方法。和 PCR 一样，tHDA 反应选择性地扩增由两个引物所确定的靶序列。然而，与 PCR 不同的是，tHDA 使用的是解旋酶将 DNA 双链分开，而不是高温变性。因此，可以在恒温条件下扩增 DNA 而不需要热循环。可用于扩增和检测 DNA 短序列（70-120 bp）。tHDA 反应还能与反转录联用而进行RNA 分析。 IsoAmp Ⅱ 通用 tHDA 试剂盒基于第二代的热解旋酶扩增平台。提供tHDA，反转录HDA（RT-HDA），实时定量 HAD（qHDA）和实时定量 RT-HAD（qRT-HAD），所有反应都采用同一种反应缓冲液。

The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes. 

（LAMP）环介导等温扩增反应是一种核酸扩增技术，广泛用于目标 DNA 或 RNA 的检测。NEB的 WarmStart® LAMP 产品线为检测目标 DNA 或 RNA提供简单、一步法的解决方案。试剂盒中提供预混液，混合有快速、强力的 Bst 2.0 WarmStart® DNA 聚合酶 和 WarmStart RTx 反转录酶，两种酶均经过基因工程改造，提高了 LAMP 反应的效率。试剂盒还提供了一种荧光染料以便于在 LAMP 反应中进行实时荧光检测。WarmStart LAMP 试剂盒可以兼容多种检测方法，包括浊度检测、实时荧光检测（当使用灯荧光染料时）和可视化终点法。The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.

全长的Bst DNA聚合酶具有5´→3´聚合酶活性和双链特异的5´→3´核酸外切酶活性，但缺失3´→5´核酸外切酶活性。用于 DNA 等温扩增， 引物延伸。建议反应温度不要超过 70℃。Bst DNA 聚合酶，全长不能用于热循环测序或 PCR。

Bst DNA Polymerase Large Fragment 是Bacillus stearothermophilus DNA聚合酶的一部分，具有 5’→3’ DNA聚合酶活性，以及很强的链置换活性，但缺失5’→3’ 核酸外切酶活性。Bst DNA Polymerase Large Fragment可用于等温扩增反应，isothermal amplification)，RCA (Rolling-circle amplification) 等。

Bst DNA Polymerase Large Fragment 是Bacillus stearothermophilus DNA聚合酶的一部分，具有 5’→3’ DNA聚合酶活性，以及很强的链置换活性，但缺失5’→3’ 核酸外切酶活性。Bst DNA Polymerase Large Fragment可用于等温扩增反应，isothermal amplification)，RCA (Rolling-circle amplification) 等。