增强的ECL化学发光检测试剂，50ml。具有更高的敏感性，可以检测低达皮克级水平的蛋白。与其他的发光系统相比较， SignalFire™ ECL 试剂可以产生更强的信号，可以更长时间的输出信号。底物暴露于HRP后，光发射立即达到更大并且可以持续0.5-1小时。光可以被记录在胶片上，一般暴露几秒钟。延长暴露时间可以增加灵敏度。 50ml(25ml each substrate). An enhanced luminol based system designed for use with CST™ HRP-linked secondary antibodies and Phototope®-HRP detection assays. SignalFire™ ECL Reagent is extremely sensitive and capable of detecting protein levels at low picogram amounts. Compared to other luminol based systems, SignalFire™ ECL Reagent boasts a more robust signal and extended duration of signal output. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained with longer exposure.
20ml。一种更灵敏的化学发光底物，可通过western blot检测低皮克级别的蛋白质。发光时间延长，可持续数小时，允许多次曝光，兼容胶片和数码成像系统。更强烈的信号输出，可检测低丰度蛋白,节省试剂,缩短曝光时间。SignalFire™ Plus ECL Reagent所需的抗体相较于传统ECL试剂要少6倍。限制与膜接触的HRP量可以防止高背景、靶蛋白信号的过饱和,或假阴性结果。其他HRP偶联抗体,包括HRP偶联二抗应进行稀释。 A highly sensitive chemiluminescent substrate capable of detecting low picogram amounts of protein by western blot analysis. SignalFire™ Plus ECL Reagent has an extended duration of signal output lasting several hours following blot exposure, allowing for multiple exposures with either film or a digital imaging system. The strong signal output allows detection of low abundance proteins, conservation of reagents, and short exposure times. 20ml.
SignalFire™ Plus ECL Reagent requires approximately five-fold less Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7077 than traditional ECL reagents. Limiting the amount of HRP exposed to the membrane prevents high background, oversaturation of the target protein signal, or false negative results. Other HRP-conjugated antibodies, including HRP-conjugated primary and anti-biotin-HRP antibodies, should be diluted similarly. Dilution of secondary antibody from alternative vendors may need to be optimized. Titration of lysate and primary antibody concentration is recommended to achieve optimal signal-to-noise ratio.
20ml。一种超级灵敏的化学发光底物，可通过western blot检测低飞克级别的蛋白质。兼容胶片和数码成像系统。更强烈的信号输出，可检测极低丰度蛋白,节省试剂,缩短曝光时间。SignalFire™ Plus ECL Reagent所需的抗体仅为传统ECL试剂的10分之一。限制与膜接触的HRP量可以防止高背景、靶蛋白信号的过饱和,或假阴性结果。其他HRP偶联抗体,包括HRP偶联二抗应进行稀释。 An ultra sensitive chemiluminescent substrate capable of detecting femtogram amounts of protein by western blot analysis. SignalFire™ Elite ECL Reagent is compatible with both film and digital imaging systems. The extremely intense signal output allows detection of very low abundance proteins, conservation of reagents, and short exposure times. 20ml.
SignalFire™ Elite ECL Reagent requires approximately ten-fold less Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7076 than traditional ECL reagents. Limiting the amount of HRP exposed to the membrane prevents high background, oversaturation of the target protein signal, or false negative results. Other HRP-conjugated antibodies, including HRP-conjugated primary and anti-biotin-HRP antibodies, should be diluted similarly. Dilution of secondary antibody from alternative vendors may need to be optimized. Titration of lysate and primary antibody concentration is recommended to achieve optimal signal-to-noise ratio. 20 ml
增强的ECL化学发光检测试剂，500ml。具有更高的敏感性，可以检测低达皮克级水平的蛋白。与其他的发光系统相比较， SignalFire™ ECL 试剂可以产生更强的信号，可以更长时间的输出信号。底物暴露于HRP后，光发射立即达到更大并且可以持续0.5-2小时。光可以被记录在胶片上，一般暴露几秒钟。延长暴露时间可以增加灵敏度。 500 ml(250 ml each substrate). An enhanced luminol based system designed for use with CST™ HRP-linked secondary antibodies and Phototope®-HRP detection assays. SignalFire™ ECL Reagent is extremely sensitive and capable of detecting protein levels at low picogram amounts. Compared to other luminol based systems, SignalFire™ ECL Reagent boasts a more robust signal and extended duration of signal output. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained with longer exposure.
50次反应。试剂盒含发光底物，HRP偶联的抗小鼠二抗和生物素标记蛋白分子量标记，灵敏度高，发光持续时间长，可检测亚皮克级的蛋白质。为标准的western blotting 应用中检测化学发光的蛋白而设计。蛋白和生物素化的分子量标记（已提供）可以在SDS-PAGE中分离，再被转移到膜上。与抗血清的一抗和辣根过氧化物酶（HRP） 标记的二抗孵育后，HRP标记的抗生物素抗体被结合，然后与LumiGLO ® 试剂反应，发出的光随后被捕获到X胶片上。 Designed for the chemiluminescent detection of proteins in standard Western blotting applications. Proteins and biotinylated molecular weight markers (provided) are separated by SDS-PAGE and transferred onto membrane. Following incubation with your primary anti-serum, horseradish peroxidase (HRP) linked secondary antibody and HRP-linked anti-biotin antibody are bound and then allowed to react with LumiGLO® reagent. The light emitted by destabilized LumiGLO® reagent is subsequently captured on X-ray film. 50 assays. 20X LumiGLO® Reagent and 20X Peroxide #7003 25 ml each
Anti-mouse IgG, HRP-linked Antibody #7076 250 µl Goat
Biotinylated Protein Ladder Detection Pack #7727 500 µl LumiGLO chemiluminescent substrate is a luminol-based system designed for use with our Phototope-HRP detection assays utilizing peroxidase-labeled antibodies immobilized on membranes. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 0.5-1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained by longer exposure. *Avoid repeated exposure to skin
50次反应。试剂盒含发光底物，HRP偶联的抗兔二抗和生物素标记蛋白分子量标记，灵敏度高，发光持续时间长，可检测亚皮克级的蛋白质。为标准的western blotting 应用中检测化学发光的蛋白而设计。蛋白和生物素化的分子量标记（已提供）可以在SDS-PAGE中分离，再被转移到膜上。与抗血清的一抗和辣根过氧化物酶（HRP） 标记的二抗孵育后，HRP标记的抗生物素抗体被结合，然后与LumiGLO ® 试剂反应，发出的光随后被捕获到X胶片上。 Designed for the chemiluminescent detection of proteins in standard Western blotting applications. Proteins and biotinylated molecular weight markers (provided) are separated by SDS-PAGE and transferred onto membrane. Following incubation with your primary anti-serum, horseradish peroxidase (HRP) linked secondary antibody and HRP-linked anti-biotin antibody are bound and then allowed to react with LumiGLO® reagent. The light emitted by destabilized LumiGLO® reagent is subsequently captured on X-ray film. 50 assays. 20X LumiGLO® Reagent and 20X Peroxide #7003 25 ml each
Anti-rabbit IgG, HRP-linked Antibody #7074 250 µl Goat
Biotinylated Protein Ladder Detection Pack #7727 500 µl LumiGLO chemiluminescent substrate is a luminol-based system designed for use with our Phototope-HRP detection assays utilizing peroxidase-labeled antibodies immobilized on membranes. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 0.5-1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained by longer exposure. *Avoid repeated exposure to skin
100ml。一种更灵敏的化学发光底物，可通过western blot检测低皮克级别的蛋白质。发光时间延长，可持续数小时，允许多次曝光，兼容胶片和数码成像系统。更强烈的信号输出，可检测低丰度蛋白,节省试剂,缩短曝光时间。SignalFire™ Plus ECL Reagent所需的抗体相较于传统ECL试剂要少5倍。限制与膜接触的HRP量可以防止高背景、靶蛋白信号的过饱和,或假阴性结果。其他HRP偶联抗体,包括HRP偶联二抗应进行稀释。 A highly sensitive chemiluminescent substrate capable of detecting low picogram amounts of protein by western blot analysis. SignalFire™ Plus ECL Reagent has an extended duration of signal output lasting several hours following blot exposure, allowing for multiple exposures with either film or a digital imaging system. The strong signal output allows detection of low abundance proteins, conservation of reagents, and short exposure times. 100ml.
SignalFire™ Plus ECL Reagent requires approximately five-fold less Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7076 than traditional ECL reagents. Limiting the amount of HRP exposed to the membrane prevents high background, oversaturation of the target protein signal, or false negative results. Other HRP-conjugated antibodies, including HRP-conjugated primary and anti-biotin-HRP antibodies, should be diluted similarly. Dilution of secondary antibody from alternative vendors may need to be optimized. Titration of lysate and primary antibody concentration is recommended to achieve optimal signal-to-noise ratio.
100ml。一种超级灵敏的化学发光底物，可通过western blot检测低飞克级别的蛋白质。兼容胶片和数码成像系统。更强烈的信号输出，可检测极低丰度蛋白,节省试剂,缩短曝光时间。SignalFire™ Plus ECL Reagent所需的抗体仅为传统ECL试剂的10分之一。限制与膜接触的HRP量可以防止高背景、靶蛋白信号的过饱和,或假阴性结果。其他HRP偶联抗体,包括HRP偶联二抗应进行稀释。 An ultra sensitive chemiluminescent substrate capable of detecting femtogram amounts of protein by western blot analysis. SignalFire™ Elite ECL Reagent is compatible with both film and digital imaging systems. The extremely intense signal output allows detection of very low abundance proteins, conservation of reagents, and short exposure times. 100ml.
SignalFire™ Elite ECL Reagent requires approximately ten-fold less Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7076 than traditional ECL reagents. Limiting the amount of HRP exposed to the membrane prevents high background, oversaturation of the target protein signal, or false negative results. Other HRP-conjugated antibodies, including HRP-conjugated primary and anti-biotin-HRP antibodies, should be diluted similarly. Dilution of secondary antibody from alternative vendors may need to be optimized. Titration of lysate and primary antibody concentration is recommended to achieve optimal signal-to-noise ratio. 21 ml
各25ml。化学发光底物，遇HRP后发光，可以持续0.5-1小时，压片时间通常数秒到数分钟，允许重复压片或者调整压片时间。灵敏度高，可检测亚皮克级的蛋白质。 LumiGLO chemiluminescent substrate is a luminol-based system designed for use with our Phototope-HRP detection assays utilizing peroxidase-labeled antibodies immobilized on membranes. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 0.5-1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained by longer exposure. *Avoid repeated exposure to skin
Santa Cruz Biotechnology Luminol reagent is a non-radioactive, light-emitting system for detecting proteins on a Western blot membrane. Horseradish peroxidase mediates oxidation of Luminol reagent in the presence of hydrogen peroxide and this reaction produces an iridescent light.Western blotting Luminol reagent is recommended for Western blotting enhanced chemiluminescence applications using Horseradish Peroxidase (HRP) conjugated secondary antibodies.One Western blotting Luminol reagent package contains 125 ml each Solution A and Solution B, which combine to form sufficient chemiluminescence reagent for a 2,000 cm squared membrane area.Extended exposure times (multiple exposures) to film made possible by long-duration signalWestern blotting Luminol reagent solutions are stable at room temperature for one hour. Store at 2-8° C for longer periods up to 12 months. Store in the dark.
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