1 ml Protein A 或 Protein G 磁珠结合 > 400 μg 人 IgG。Protein A磁珠是一种能用于少量分离纯化免疫球蛋白的亲和介质。它们对来源于许多哺乳动物种属的 IgG 亚型具有较高亲和力，如：人、兔和小鼠。本品是将重组的截短型 Protein A 共价耦联到一种无孔的顺磁颗粒上而形成的。截短型蛋白展示了对 IgG Fc 区更高的单位结合力和降低的非特异结合力。Protein A对各种 IgG 的亲和性随物种不同和同一种属 IgG 亚系不同而不同。
由于 Protein A 和 Protein G 与磁珠的耦联在较宽的 pH 范围内都是稳定且致密的，因此可以对来自腹水、血清或细胞培养上清物中的 IgG 进行磁免疫纯化，介质随后还可以进行再生而不影响结合容量。它们还可用于免疫沉淀法，用所选一抗将目的蛋白从细胞粗提液中沉淀。此外，利用 Protein A 或 Protein G 磁珠还能制成可重复使用的免疫共沉淀磁珠。特定的抗体通过化学键交联到被 Protein A 或 Protein G 包被的磁珠表面，形成一个可重复使用的免疫共沉淀磁珠，避免了抗体与目的抗原共同洗脱。
Protein G 磁珠是一种能用于少量分离纯化免疫球蛋白的亲和介质。它们对来源于许多哺乳动物种属的 IgG 亚型具有较高亲和力，如：人、兔和小鼠。本品是将重组的截短型Protein G共价耦联到一种无孔的顺磁颗粒上而形成的。截短型蛋白展示了对 IgG Fc 区更高的单位结合力和降低的非特异结合力。Protein G 对各种 IgG 的亲和性随物种不同和同一种属 IgG 亚系不同而不同。
由于 Protein A 和 Protein G 与磁珠的耦联在较宽的 pH 范围内都是稳定且致密的，因此可以对来自腹水、血清或细胞培养上清物中的 IgG 进行磁免疫纯化，介质随后还可以进行再生而不影响结合容量。它们还可用于免疫沉淀法，用所选一抗将目的蛋白从细胞粗提液中沉淀。此外，利用 Protein A 或 Protein G 磁珠还能制成可重复使用的免疫共沉淀磁珠。特定的抗体通过化学键交联到被 Protein A 或 Protein G 包被的磁珠表面，形成一个可重复使用的免疫共沉淀磁珠，避免了抗体与目的抗原共同洗脱。
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-TED 1000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-TED 1000 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-IDA 1000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-IDA 1000 Packed Columns, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-TED 2000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-TED 2000 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-IDA 2000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-IDA 2000 Packed Columns, buffers
Ni-TED纯化填料螯合Ted基团，可耐受高压高流速，可用于FPLC，高亲和高选择性得到更纯的His-tag蛋白，在还原和螯合试剂中亦能保持高度稳定，极低金属离子解离。干粉形式提供更易保存。有配套的空纯化柱和96孔纯化板提供，灵活多用。 High specificity for Purification of His-tag proteins, the purest alternative for His-tag protein purification • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Dry resin → storage at room temperature
Protino® Ni-TED Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility • Batch procedure, gravity-flow column chromatography, FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-TED Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin
Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield / recovery even from diluted samples • High protein concentration • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Dry resin → storage at room temperature
Protino® Ni-IDA Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility • Batch procedure, gravity-flow column chromatography, FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-IDA Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin
Technology: IMAC, immobilized metal ion affinity chromatography Chelating ligand: IDA (iminodiacetic acid) Format: Bulk matrix Matrix: Macroporous silica Physical form: Dry matrix, precharged with Ni2+ Binding capacity*: 20 mg/g resin, 10 mg/mL bed volume Max. pressure 145 psi (10 bar)
* Binding capacity will vary for each polyhistidin-tagged protein
Applications • Purification of polyhistidine-tagged proteins • Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield / recovery even from diluted samples • High protein concentration • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Dry resin → storage at room temperature
Protino® Ni-IDA Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility • Batch procedure, gravity-flow column chromatography, FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-IDA Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin
Technology: IMAC, immobilized metal ion affinity chromatography Chelating ligand: IDA (iminodiacetic acid) Format: Bulk matrix Matrix: Macroporous silica Physical form: Dry matrix, precharged with Ni2+ Binding capacity*: 20 mg/g resin, 10 mg/mL bed volume Max. pressure 145 psi (10 bar)
* Binding capacity will vary for each polyhistidin-tagged protein
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-IDA 150 Packed Columns, buffers 10 preps for the purification of His-tag proteins - Protino Ni-IDA 150 Packed Columns, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-TED 150 Packed Columns, buffers 10 preps for the purification of His-tag proteins - Protino Ni-TED 150 Packed Columns, buffers buffers
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