1 ml Protein A 或 Protein G 磁珠结合 > 400 μg 人 IgG。Protein A磁珠是一种能用于少量分离纯化免疫球蛋白的亲和介质。它们对来源于许多哺乳动物种属的 IgG 亚型具有较高亲和力，如：人、兔和小鼠。本品是将重组的截短型 Protein A 共价耦联到一种无孔的顺磁颗粒上而形成的。截短型蛋白展示了对 IgG Fc 区更高的单位结合力和降低的非特异结合力。Protein A对各种 IgG 的亲和性随物种不同和同一种属 IgG 亚系不同而不同。
由于 Protein A 和 Protein G 与磁珠的耦联在较宽的 pH 范围内都是稳定且致密的，因此可以对来自腹水、血清或细胞培养上清物中的 IgG 进行磁免疫纯化，介质随后还可以进行再生而不影响结合容量。它们还可用于免疫沉淀法，用所选一抗将目的蛋白从细胞粗提液中沉淀。此外，利用 Protein A 或 Protein G 磁珠还能制成可重复使用的免疫共沉淀磁珠。特定的抗体通过化学键交联到被 Protein A 或 Protein G 包被的磁珠表面，形成一个可重复使用的免疫共沉淀磁珠，避免了抗体与目的抗原共同洗脱。
Protein G 磁珠是一种能用于少量分离纯化免疫球蛋白的亲和介质。它们对来源于许多哺乳动物种属的 IgG 亚型具有较高亲和力，如：人、兔和小鼠。本品是将重组的截短型Protein G共价耦联到一种无孔的顺磁颗粒上而形成的。截短型蛋白展示了对 IgG Fc 区更高的单位结合力和降低的非特异结合力。Protein G 对各种 IgG 的亲和性随物种不同和同一种属 IgG 亚系不同而不同。
由于 Protein A 和 Protein G 与磁珠的耦联在较宽的 pH 范围内都是稳定且致密的，因此可以对来自腹水、血清或细胞培养上清物中的 IgG 进行磁免疫纯化，介质随后还可以进行再生而不影响结合容量。它们还可用于免疫沉淀法，用所选一抗将目的蛋白从细胞粗提液中沉淀。此外，利用 Protein A 或 Protein G 磁珠还能制成可重复使用的免疫共沉淀磁珠。特定的抗体通过化学键交联到被 Protein A 或 Protein G 包被的磁珠表面，形成一个可重复使用的免疫共沉淀磁珠，避免了抗体与目的抗原共同洗脱。
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-TED 1000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-TED 1000 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-IDA 1000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-IDA 1000 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-IDA 2000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-IDA 2000 Packed Columns, buffers
Ni-TED纯化填料螯合Ted基团，可耐受高压高流速，可用于FPLC，高亲和高选择性得到更纯的His-tag蛋白，在还原和螯合试剂中亦能保持高度稳定，极低金属离子解离。干粉形式提供更易保存。有配套的空纯化柱和96孔纯化板提供，灵活多用。High specificity for Purification of His-tag proteins, the purest alternative for His-tag protein purification• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices• Dry resin → storage at room temperature Protino® Ni-TED Resin• Dry bulk material• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility• Batch procedure, gravity-flow column chromatography, FPLC™ applications• Empty Protino® columns available for use with Protino® Ni-TED Resin• Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin Technology: IMAC (immobilized metal ion affinity chromatography) Chelating ligand: TED (tris-carboxymethyl ethylene diamine) Format: Bulk matrix Matrix: Macroporous silica Physical form: Dry matrix, precharged with Ni2+ Binding capacity* 10 mg/g resin, 5 mg/mL bed volumeMax. pressure 145 psi (10 bar) * Binding capacity will vary for each polyhistidin-tagged protein Applications • Purification of polyhistidine-tagged proteins• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications Protino Ni-TED Resin for the purification of His-tag proteins pack of 5 g
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-TED 2000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-TED 2000 Packed Columns, buffers
Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield / recovery even from diluted samples• High protein concentration• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices• Dry resin → storage at room temperature Protino® Ni-IDA Resin• Dry bulk material• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility• Batch procedure, gravity-flow column chromatography, FPLC™ applications• Empty Protino® columns available for use with Protino® Ni-IDA Resin• Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin Technology: IMAC, immobilized metal ion affinity chromatographyChelating ligand: IDA (iminodiacetic acid)Format: Bulk matrixMatrix: Macroporous silicaPhysical form: Dry matrix, precharged with Ni2+Binding capacity*: 20 mg/g resin, 10 mg/mL bed volumeMax. pressure 145 psi (10 bar) * Binding capacity will vary for each polyhistidin-tagged protein Applications • Purification of polyhistidine-tagged proteins• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield / recovery even from diluted samples• High protein concentration• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices• Dry resin → storage at room temperature Protino® Ni-IDA Resin• Dry bulk material• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility• Batch procedure, gravity-flow column chromatography, FPLC™ applications• Empty Protino® columns available for use with Protino® Ni-IDA Resin• Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin Technology: IMAC, immobilized metal ion affinity chromatographyChelating ligand: IDA (iminodiacetic acid)Format: Bulk matrixMatrix: Macroporous silicaPhysical form: Dry matrix, precharged with Ni2+Binding capacity*: 20 mg/g resin, 10 mg/mL bed volumeMax. pressure 145 psi (10 bar) * Binding capacity will vary for each polyhistidin-tagged protein Applications • Purification of polyhistidine-tagged proteins• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications Protino Ni-IDA Resin for the purification of His-tag proteins pack of 5 g
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-IDA 150 Packed Columns, buffers 10 preps for the purification of His-tag proteins - Protino Ni-IDA 150 Packed Columns, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-TED 150 Packed Columns, buffers 10 preps for the purification of His-tag proteins - Protino Ni-TED 150 Packed Columns, buffers buffers
预装FPLC柱。预装Ni-NTA Agarose，用于纯化His-tag蛋白。Ready-to-use FPLC™ columns filled with Protino® Ni-NTA AgaroseApplications• Purification of polyhistidine-tagged proteins• Compatible with automated liquid chromatography systems (MPLC, FPLC™, ÄKTAdesign™, etc.)FPLC™ columns FPLC column (5 mL) for the purification of His-tag proteins pack of 1
预装FPLC柱。预装Ni-NTA Agarose，用于纯化His-tag蛋白。Ready-to-use FPLC™ columns filled with Protino® Ni-NTA AgaroseApplications• Purification of polyhistidine-tagged proteins• Compatible with automated liquid chromatography systems (MPLC, FPLC™, ÄKTAdesign™, etc.)FPLC™ columns FPLC columns (1 mL) for the purification of His-tag proteins pack of 5
预装Ni-IDA的96孔预装板，高通量纯化His-tag蛋白。• Chelating group IDA (iminodiacetic acid), three protein binding sites• High protein yield/recovery even from diluted samples• High protein concentration• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Ready-to-use prepacked 96-well plates filled with dry Protino® Ni-IDA Resin ® storage at room temperature• Buffers included Technology: IMAC (immobilized metal ion affinity chromatography) Chelating group: IDA (iminodiacetic acid) Format: ready-to-use 96-well plates Matrix: Macroporous silicaPhysical form: Dry matrix, precharged with Ni2+ Amount of resin per well: 50 mg Bed volume: 100 µL Binding capacity*: 1 mg/well (* refers to 6xHis-GFPuv) Applications • Purification of polyhistidine-tagged proteins• 96-well format gravity flow chromatography Protino® 96 Ni-IDA Plates, buffers 1 x 96 preps for the purification of His-tag proteins - Protino Ni-IDA Plate, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-TED 150 Packed Columns, buffers 50 preps for the purification of His-tag proteins - Protino Ni-TED 150 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-IDA 150 Packed Columns, buffers 50 preps for the purification of His-tag proteins - Protino Ni-IDA 150 Packed Columns, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-TED 1000 Packed Columns, buffers 50 preps for the purification of His-tag proteins - Protino Ni-TED 1000 Packed Columns, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-TED 2000 Packed Columns, buffers 25 preps for the purification of His-tag proteins - Protino Ni-TED 2000 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-IDA 1000 Packed Columns, buffers 50 preps for the purification of His-tag Proteins - Protino Ni-IDA 1000 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin• Buffers included• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matricesProtino® Ni-IDA 2000 Packed Columns, buffers 25 preps for the purification of His-tag proteins - Protino Ni-IDA 2000 Packed Columns, buffers
更具性价比的GST-tag蛋白亲和纯化填料，适用范围广• Aqueous suspension, the cost-effective alternative for GST-tag protein purification • For batch binding, gravity flow, and FPLC™ → highest flexibility of applications• Empty Protino® columns for gravity flow available• Simply replace your current products, no optimization or protocol change necessary• Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use• Market-leading performance for an unbeatable priceTechnology Affinity chromatography Chelating ligand Glutathione, linked via sulfur atom Format 75 % (v/v) aqueous suspension containing 20 % ethanol Matrix: 4 % beaded agarose Bead size: 90 µm Binding capacity* :> 8 mg/mLRecommended flow rates:1 mL bed volume (column with 6.6 mm inner diameter): Sample loading**: 0.2–1.0 mL/min. Washing / elution: 1.0 mL/min 10 mL bed volume (column with 16 mm inner diameter): Sample loading**: 0.5–5.0 mL/min. Washing / elution: 5.0 mL/min Max. linear flow rate 250 cm/h Chemical stability: Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate pH 4, 0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yieldStorage temperature 4–8 °C Note Do not autoclave the gel * Binding capacity will vary for each GST-tagged protein ** Slow flow rates are recommended for the loading step to allow maximal binding of the GST-tagged protein Applications • Purification of GST-tagged proteins• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications Protino Glutathione Agarose 4B suspension for the purification of GST-tag proteins pack of 10 mL settled beaded agarose
即用型FPLC预装柱，预装Protino® Glutathione Agarose 4B，用于亲和纯化GST-tag蛋白Ready-to-use FPLC™ columns filled with Protino® Glutathione Agarose 4B，the cost-effective alternative for GST-tag protein purification • Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns• Simply replace your current products, no optimization or protocol change necessary• Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use• Market-leading performance for an unbeatable price FPLC columns (1 mL) for purification of GST-tag proteins pack of 5
Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield / recovery even from diluted samples• High protein concentration• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices• Dry resin → storage at room temperature Protino® Ni-IDA Resin• Dry bulk material• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility• Batch procedure, gravity-flow column chromatography, FPLC™ applications• Empty Protino® columns available for use with Protino® Ni-IDA Resin• Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin Technology: IMAC, immobilized metal ion affinity chromatographyChelating ligand: IDA (iminodiacetic acid)Format: Bulk matrixMatrix: Macroporous silicaPhysical form: Dry matrix, precharged with Ni2+Binding capacity*: 20 mg/g resin, 10 mg/mL bed volumeMax. pressure 145 psi (10 bar) * Binding capacity will vary for each polyhistidin-tagged protein Applications • Purification of polyhistidine-tagged proteins• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield / recovery even from diluted samples• High protein concentration• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices• Dry resin → storage at room temperature Protino® Ni-IDA Resin• Dry bulk material• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility• Batch procedure, gravity-flow column chromatography, FPLC™ applications• Empty Protino® columns available for use with Protino® Ni-IDA Resin• Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin Technology: IMAC, immobilized metal ion affinity chromatographyChelating ligand: IDA (iminodiacetic acid)Format: Bulk matrixMatrix: Macroporous silicaPhysical form: Dry matrix, precharged with Ni2+Binding capacity*: 20 mg/g resin, 10 mg/mL bed volumeMax. pressure 145 psi (10 bar) * Binding capacity will vary for each polyhistidin-tagged protein Applications • Purification of polyhistidine-tagged proteins• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications Protino Ni-IDA Resin for the purification of His-tag proteins pack of 30 g
Ni-TED纯化填料螯合Ted基团，可耐受高压高流速，可用于FPLC，高亲和高选择性得到更纯的His-tag蛋白，在还原和螯合试剂中亦能保持高度稳定，极低金属离子解离。干粉形式提供更易保存。有配套的空纯化柱和96孔纯化板提供，灵活多用。High specificity for Purification of His-tag proteins, the purest alternative for His-tag protein purification• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents• Low metal leaching• High protein purity• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices• Dry resin → storage at room temperature Protino® Ni-TED Resin• Dry bulk material• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility• Batch procedure, gravity-flow column chromatography, FPLC™ applications• Empty Protino® columns available for use with Protino® Ni-TED Resin• Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin Technology: IMAC (immobilized metal ion affinity chromatography) Chelating ligand: TED (tris-carboxymethyl ethylene diamine) Format: Bulk matrix Matrix: Macroporous silica Physical form: Dry matrix, precharged with Ni2+ Binding capacity* 10 mg/g resin, 5 mg/mL bed volumeMax. pressure 145 psi (10 bar) * Binding capacity will vary for each polyhistidin-tagged protein Applications • Purification of polyhistidine-tagged proteins• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications Protino Ni-TED Resin for the purification of His-tag proteins pack of 30 g
性价比的His-tag蛋白亲和纯化填料。性能出众价格低廉The cost-effective NTA products for His-tag protein purification. Leading performance, unbeatable price• Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies)• High capacity and high affinity• Simply replace your current Ni-NTA products, no optimization or protocol change necessary• Purification under native and denaturing conditions• Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use• Market-leading performance for an unbeatable priceProtino® Ni-NTA Agarose• Aqueous suspension• For batch binding, gravity flow, and FPLC™ → highest flexibility of applications• Empty Protino® Columns for gravity flow available Technology: IMAC (immobilized metal ion affinity chromatography)Chelating ligand NTA (nitrilotriacetic acid) Format: 50 % (v/v) aqueous suspension containing 30 % ethanolMatrix: 6 % beaded agarose (cross-linked), precharged with Ni2+Bead size: 45–165 µmBinding capacity* ≤ 50 mg/mL Storage temperature: 4–8 °C * Binding capacity will vary for each polyhistidine-tagged protein Protino Ni-NTA suspension for the purification of His-tag proteins pack of 25 mL settled beaded agarose
即用型FPLC预装柱，预装Protino® Glutathione Agarose 4B，用于亲和纯化GST-tag蛋白Ready-to-use FPLC™ columns filled with Protino® Glutathione Agarose 4B，the cost-effective alternative for GST-tag protein purification • Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns• Simply replace your current products, no optimization or protocol change necessary• Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use• Market-leading performance for an unbeatable price FPLC column (5 mL) for purification of GST-tag proteins pack of 1
预装FPLC柱。预装Ni-NTA Agarose，用于纯化His-tag蛋白。Ready-to-use FPLC™ columns filled with Protino® Ni-NTA AgaroseApplications• Purification of polyhistidine-tagged proteins• Compatible with automated liquid chromatography systems (MPLC, FPLC™, ÄKTAdesign™, etc.)as above FPLC columns (5 mL) for the purification of His-tag proteins pack of 5
预装Ni-IDA的96孔预装板，高通量纯化His-tag蛋白。• Chelating group IDA (iminodiacetic acid), three protein binding sites• High protein yield/recovery even from diluted samples• High protein concentration• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Ready-to-use prepacked 96-well plates filled with dry Protino® Ni-IDA Resin ® storage at room temperature• Buffers included Technology: IMAC (immobilized metal ion affinity chromatography) Chelating group: IDA (iminodiacetic acid) Format: ready-to-use 96-well plates Matrix: Macroporous silicaPhysical form: Dry matrix, precharged with Ni2+ Amount of resin per well: 50 mg Bed volume: 100 µL Binding capacity*: 1 mg/well (* refers to 6xHis-GFPuv) Applications • Purification of polyhistidine-tagged proteins• 96-well format gravity flow chromatography Protino® 96 Ni-IDA Plates, buffers 4 x 96 preps for the purification of His-tag proteins - Protino Ni-IDA Plates, buffers
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