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生物通商城首页 -> 试剂 > 免疫学 > AlphaLISA系列 > AlphaLISA表观遗传学工具

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通过表观遗传酶来测量肽和蛋白质修饰,高灵敏度且无需洗涤。

AlphaLISA 技术可用于检测表观遗传研究的特定甲基化和乙酰化状态。高质量的数据、可靠的性能、简单、可快速优化。AlphaLISA 技术是无需洗涤的均相亲近免疫检测技术,其中包括定量组蛋白、肽或蛋白质基质活性的 Alpha 供体微珠和 AlphaLISA 受体微珠。培养酶(如组蛋白乙酰转移酶或组蛋白甲基转移酶)利用 S-腺苷甲硫氨酸 (SAM) 或乙酰辅酶 A 辅助因子和组蛋白基质,对基质进行体外甲基化或乙酰化。在酶反应后,将使用特定标记的抗-甲基或抗-乙酰基结合抗体 AlphaLISA 受体微珠来监测甲基化和乙酰化。添加包被有链霉亲和素的供体微珠以捕获基质后,微珠将相互趋近。供体微珠受到波长为 680nm 的激光激发而释放单线态氧分子。单线态氧分子引发受体微珠的反应,并发射波长为 615 nm 的光。其发射强度与甲基化或乙酰化的基质量成比例。 



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产品名/货号/品牌 简介 包装  
Alpha乙酰化的组蛋白H3(H3K27ac)定量检测试剂盒:AlphaLISA H3K27ac cellular detection kit 100次(数据点)

价格:¥

货号:AL720HV
品牌:PERKINELMER

经销:基因有限公司

Alpha甲基化的组蛋白H3(H3K27me2-1)定量检测试剂盒:AlphaLISA H3K27me2-1 cellular detection kit 100次(数据点)

价格:¥

货号:AL721HV
品牌:PERKINELMER

经销:基因有限公司

Alpha甲基化的组蛋白H3(H3K36me2)定量检测试剂盒:AlphaLISA H3K36me2 cellular detection kit 100次(数据点)

价格:¥

货号:AL723HV
品牌:PERKINELMER

经销:基因有限公司

The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput. Simple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns Suitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models Ideal for HTS protocols with demonstrated Z’ factors greater than 0.6 The AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Alpha乙酰化的组蛋白H3(H3K9ac)定量检测试剂盒:AlphaLISA H3K9ac cellular detection kit 100次(数据点)

价格:¥

货号:AL714HV
品牌:PERKINELMER

经销:基因有限公司

The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput. Simple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns Suitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models Ideal for HTS protocols with demonstrated Z’ factors greater than 0.6 The AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

生物素标记的抗组蛋白H3 C末端抗体 :Biotinylated anti-Histone H3 (C-terminal) ant 2 µg

价格:¥

货号:AL118C
品牌:PerkinElmer

经销:基因有限公司