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生物通商城首页 > 试剂 > 表观遗传学 > AlphaLISA表观遗传学工具

表观遗传学

DNA修饰的检测定量

甲基化敏感酶/相关酶

组蛋白/DNA甲基转移酶

组蛋白分析

重亚硫酸盐处理的PCR

染色质免疫共沉淀

甲基化DNA免疫沉淀

表观遗传学相关抗体

AlphaLISA表观遗传学工具

DNA对照和辅助

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不限 PerkinElmer
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分类简介:AlphaLISA technology is a no-wash homogeneous proximity immunoassay that includes Alpha Donor and AlphaLISA Acceptor beads to quantitate activity on histone, peptide, or protein substrates. In vitro methylation or acetylation of substrate is performed by incubating enzyme (for example, a histone acetyltransferase or methyltransferase) with S-adenosylmethionine (SAM) or Acetyl CoA cofactor and histone substrate. Following the enzymatic reaction, methylation or acetylation is monitored using mark-specific anti-methyl or anti-acetyl antibody-conjugated AlphaLISA Acceptor beads. Once streptavidin-coated Donor beads are added to capture the substrate, the beads are brought into close proximity. The Donor beads are excited by a laser at 680 nm to release singlet oxygen. The singlet oxygen causes a reaction in the Acceptor bead and light emission is produced at 615 nm. This emission is proportional to the amount of methylated or acetylated substrate present. 
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