高滴度的CRISPR/dCas9慢病毒激活粒子 高滴度的CRISPR/dCas9慢病毒激活粒子；Prohibitin CRISPR Activation Plasmid (h)是一种协同激活介质（SAM）转录激活系统，通过细胞慢病毒转导，特异高效地上调基因表达；Prohibitin CRISPR Activation Plasmid (h)包含以下SAM激活元素：一种是去活化的Cas9 (dCas9)核酸酶(D10A and N863A)，它融合了转录活化域VP64；一种是MS2-p65-HSF1融合蛋白；一种是目标特定的20 nt向导RNA。它们还含有杀稻瘟菌素，潮霉素和嘌呤霉素抗性基因；一经转导，形成的SAM复合物结合到转录起始位点上游大约200-250 nt的特定位点区域，为高效激活基因，招募转录因子；基因激活效率可用抗体通过WB, IF或者IHC测定: Prohibitin (E-5): sc-377037或通过WB, IF, 或IHC用抗体: Prohibitin (H-80): sc-28259 200 μl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles Prohibitin Lentiviral Activation Particles (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells Prohibitin Lentiviral Activation Particles (h) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt. guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt. upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation Gene activation efficiency can be assayed by WB, IF or IHC using antibody: Prohibitin (E-5): sc-377037 or by WB, IF or IHC using antibody: Prohibitin (H-80): sc-28259
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