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          生物通商城首页 > 试剂 > 分子生物学 > 报告系统/蛋白标记定位 > SNAP-Cell

          SNAP-Cell 430, 50 nmol

          品牌:NEB 货号:S9109S 立即咨询 QQ 咨询留言

          商城价:

          ¥3102.00目录价:¥3649.00

          特价促销:

          ¥3102.00
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          • 基因生物技术国际贸易(上海)有限公司 发货并提供售后服务

          Shelf Life(month):24
          产地:USA
          存储条件:-20
          细胞通透性底物(SNAP-Cell)不仅能标记细胞内部也能标记细胞表面。激发光421nm,发射光444和484 nm。SNAP-Cell® 430 is a blue fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro. This cell-permeable substrate (BG-430) is based on diethylaminocoumarin and is suitable for appropriate blue lasers and filter sets. It has an excitation maximum at 421 nm and emission maxima at 444 and 484 nm. This package includes 50 nmol of SNAP-Cell 430 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

          The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on human O6-alkylguanine-DNA alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

          There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.
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          • 咨询标题:[咨询产品]NEB / S9109S / SNAP-Cell 430, 50 nmol
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