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          生物通商城首页 > 试剂 > 分子生物学 > 报告系统/蛋白标记定位 > SNAP-Surfase

          SNAP-Surface Alexa Fluor® 488

          SNAP-Surface Alexa Fluor 488, 50 nmol

          品牌:NEB 货号:S9129S 立即咨询 QQ 咨询留言

          商城价:

          ¥3425.00目录价:¥4029.00

          特价促销:

          ¥3425.00
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          • 基因生物技术国际贸易(上海)有限公司 发货并提供售后服务

          Shelf Life(month):24
          产地:USA
          存储条件:-20
          细胞非通透性底物(SNAP-Surface)仅能特异性的标记在细胞表面表达的融合蛋白。激发光496nm,发射光520nm。SNAP-Surface® Alexa Fluor® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate is based on BG-Alexa Fluor 488 and is suitable for standard fluorescein filter sets. It has an excitation maximum at 496 nm and emission maximum at 520 nm. This package includes 50 nmol of SNAP-Surface Alexa Fluor 488 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

          The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

          There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.
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